INHIBITORY EFFECT OF SUBEROGORGIN ON ACETYLCHOLINESTERASE

AIM: To study the selection, reversibility, and kinetics of suberogorg in (Sub) on acetylcholinesterase (AChE). METHODS: The human plasma was used as butyrylcholinesterase (BudhE). The activity of ChE was determ ined with colorimetry. RESULTS: Sub obviously inhibited the AChE in vi tro with p/(50) 4.03, 4.92, 3.82, and 4.67 in RBC membranes (of rat an d human) and tissue extracts (of rat brain and earthworm dorsal muscle ), respectively. No inhibition on BuChE was observed. The inhibition o f Sub on AChE was far lower than that of physostigmine, but was close to that of galanthamine. Sub decreased the AGhE activity to the lowest within 3 min after it was incubated with AChE. Centrifugalization was hing reactivated the AChE which had been inhibited by Sub. The double- reciprocal plots of different concentrations of Sub on AChE showed par allel lines. CONCLUSION: Sub was a selective, reversible, and contra-c ompetetive inhibitor of AChE at the binding site on the peripheral ani on region of AChE.