RAPID-DETERMINATION OF GLUFOSINATE, GLYPHOSATE AND AMINOMETHYLPHOSPHONIC ACID IN ENVIRONMENTAL WATER SAMPLES USING PRECOLUMN FLUOROGENIC LABELING AND COUPLED-COLUMN LIQUID-CHROMATOGRAPHY
Jv. Sancho et al., RAPID-DETERMINATION OF GLUFOSINATE, GLYPHOSATE AND AMINOMETHYLPHOSPHONIC ACID IN ENVIRONMENTAL WATER SAMPLES USING PRECOLUMN FLUOROGENIC LABELING AND COUPLED-COLUMN LIQUID-CHROMATOGRAPHY, Journal of chromatography, 737(1), 1996, pp. 75-83
Citations number
30
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
The approach presented in recent work [J.V. Sancho et al., J. Chromato
gr. A, 678 (1994) 59] concerning the rapid determination of glufosinat
e in environmental water samples was successfully applied to the devel
opment of efficient procedures including the assay of glyphosate and i
ts main metabolite, aminomethylphosphonic acid (AMPA). The methodology
involves two approaches: (i) a multi-residue method allowing the simu
ltaneous determination of the three analytes in environmental water sa
mples to a level of 1 mu g/l or (ii) single residue methods focused on
the analysis of a single analyte to the sub-mu g/l level. The procedu
res involve a precolumn derivatisation step with 9-fluorenylmethylchlo
roformate (FMOC-Cl) yielding highly fluorescent derivatives of the ana
lytes which then can be determined by coupled-column LC with fluoresce
nce detection using a reversed-phase C-18 column (C-1) coupled to a we
ak ion-exchange column (C-2). The separation power of the first column
(C-1) was used to achieve sensitivity, by injecting large volume samp
les, and automated sample clean-up was achieved by removing the less p
olar interferences, including the excess of hydrolysed reagent (FMOC-O
H). Using these procedures, glufosinate, glyphosate and AMPA were succ
essfully recovered from water samples at 0.50-10 mu g/l fortification
levels, with a sample throughput of at least 40 samples per day.