HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF C-14-LABELEDTERBUTHYLAZINE AND PRINCIPAL DEGRADATES IN PERCOLATION WATER AND SOILEXTRACTS FROM LEACHING EXPERIMENTS
O. Schlegel et al., HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF C-14-LABELEDTERBUTHYLAZINE AND PRINCIPAL DEGRADATES IN PERCOLATION WATER AND SOILEXTRACTS FROM LEACHING EXPERIMENTS, Journal of chromatography, 737(1), 1996, pp. 101-107
Citations number
22
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A new very porous polystyrene-divinylbenzene copolymer with large surf
ace area as an adsorber for solid-phase extraction (SPE), was used for
the analyses of terbuthylazine and principal degradates. It showed go
od recovery rates even for the dealkylated hydroxy metabolites. The po
lymer was treated similarly to a reversed-phase material, samples of 1
000 ml showed no breakthrough for most of the terbuthylazine degradate
s. A detection limit of 0.1 mu g/l could be reached even for the most
polar compounds (de-tert.-butyldeethylterbuthylazine and de-tert.-buty
lhydroxyterbuthylazine) with recovery rates of 22 and 30%. Percolated
water and soil extracts from column experiments, obtained with water a
nd methanol, were analysed using the copolymer sorbent. Analyses were
performed on a HPLC system with UV-Vis detection and a scintillation c
ounter. The compounds were separated on a RP-8 column with an acetonit
rile-phosphate-buffer gradient. In addition to terbuthylazine applied
2 months prior to extraction, deethyl- and hydroxyterbuthylazine were
detected in the soil extracts; de-tert.-butylterbuthylazine was found
only in traces.