CHRONIC EFFECT OF ALUMINUM INGESTION ON BONE IN CALCIUM-DEFICIENT RATS

In order to elucidate the relationship of osteogenesis with aluminum a nd iron deposition, we investigated the histopathological findings of bone in calcium and/or aluminium-deficient rats,together with levels o f calcium, aluminium and iron in sera and bone tissues, and also the l evel of serum parathyroid hormone. Four week old male STD-Wistar rats were divided into four groups to examine the effects of four kinds of diets for ten weeks. The rats on normal diet (Group I) and normal diet + aluminum (Group II) did not show any pathological changes of the bo nes, but in both calcium-deficient diet group (Group III) and calcium deficient diet added aluminium (Group IV), the compact bone converted into spongy bone in varying degrees, paticularly in Group IV. Aluminiu m deposition was demonstrated at the calcification fronts and the ceme nt lines only in Group TV as red or violet-red lines with aluminium st ain, together with iron deposition as revealed with Berlin blue stain, showing similar distribution pattern as aluminum. It was clearly indi cated that aluminium and iron, instead of calcium, deposited on the ca lcification front of the bone under the condition of calcium deficienc y, inhibiting the normal osteogenesis.