USE OF RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) FOR IDENTIFICATION OF GAEUMANNOMYCES SPECIES

Randomly amplified polymorphic DNAs (RAPD) were used to distinguish am ong isolates of Gaeumannomyces graminis var. tritici, G. g. var. grami nis, G. g. var. avenae, G. incrustans, and G. cylindrosporous. Of 60 r andom sequence decamer primers tested, 28 produced bands and 15 showed evidence of polymorphism. Four primers produced DNA amplification pat terns that were used to distinguish G. graminis, G. incrustans, and G. cylindrosporous. Banding patterns were similar within isolates of G. g. var. tritici, G. g. var. avenae and G. incrustans. G. g. var. grami nis showed greater variability in banding patterns. The unweighted pai r group method with arithmetical averages (UPGMA) indicated that G. g. var. tritici isolates were more closely related to G. g. var. avenae than to G. g. var. graminis isolates. The results of our study showed that RAPD markers can be used to confirm the identification of Gaeuman nomyces species and varieties. Copyright (C) 1996 Published by Elsevie r Science Ltd