Yc. Choi et al., MOLECULAR-CLONING OF MOUSE SOMATIC AND TESTIS-SPECIFIC H2B HISTONE GENES CONTAINING A METHYLATED CPG ISLAND, DNA and cell biology, 15(6), 1996, pp. 495-504
We have isolated a mouse testis-specific H2B histone gene based on the
unusual methylation of the CpG island of rat testis-specific H2B gene
in somatic tissues, After digestion of genomic DNA with the methylati
on-sensitive restriction enzyme Hha I, we found that, among 10-20 copi
es of mouse H2B histone genes, at least three copies are methylated in
somatic tissues, but not in testis, Cloning and sequence analysis of
two methylated H2B genes revealed that one gene, MTH2B, is strikingly
similar to the testis-specific histone H2B (TH2B) gene of rat and the
other, psH2B, is a pseudogene of the somatic-type H2B gene, Northern b
lot analysis revealed that the expression of the MTH2B gene is testis-
specific. During spermatogenesis, the MTH2B gene is expressed predomin
antly in pachytene spermatocytes, as observed in the expression of rat
TH2B gene, Interestingly, the MTH2B gene is largely unmethylated in e
mbryonic stem cells, but methylated in F9 embryonal carcinoma cells, T
he psH2B pseudogene is methylated in somatic tissues and F9 cells, but
only partially methylated in embryonic stem cells, Methylation of the
psH2B pseudogene seems to be attributed to its location within the co
ntext of repetitive sequences including the B1 element. The unmethylat
ion of both H2B histone genes in the testis explains how CpG islands o
f those histone genes can be maintained during evolution despite heavy
methylation in somatic tissues.