THE USE OF INVERTEBRATE PEPTIDE TOXINS TO ESTABLISH CA2-CA1 NEUROTRANSMISSION IN RAT HIPPOCAMPAL SLICES( CHANNEL IDENTITY OF CA3)

The relative contribution(s) of different Ca2+ channel subtypes to syn aptic transmission between Schaffer collaterals of hippocampal CA3 pyr amidal cells and CA1 pyramidal cell dendrites has been assessed using the synthetic invertebrate peptide toxins omega-conotoxin GVIA to bloc k N-type Ca2+ channels, omega-agatoxin-IVA to block P-type Ca2+ channe ls and omega-conotoxin MVIIC to block N-, P- and Q-type Ca2+ channels. omega-Agatoxin-IVA, omega-conctoxin GVIA and omega-conotoxin MVIIC al l produced dose-dependent inhibitions of the excitatory post-synaptic field potential (fEPSP) recorded from the CA1 region of transverse hip pocampal slices. Application of 300 nM omega-conotoxin GVIA generally produced no further inhibition to that observed with 100 nM, resulting in a maximal 50% inhibition of the fEPSP. By contrast, 30 nM omega-ag atoxin-IVA reduced the fEPSP slope by only 4.6 +/- 11.1% (mean +/- S.D ., n = 3), suggesting the lack of involvement of classical P-type Ca2 channels, whereas 300 nM omega-agatoxin-IVA reduced the fEPSP slope b y 85.7 +/- 15.3% (n = 3) at the end of 44 min application. Similar app lications of 100 and 300 nM omega-conotoxin MVIIC reduced the fEPSP sl ope by 30.9 +/- 6.6% and 79.7 +/- 5.7% respectively. Application of 30 nM omega-agatoxin-IVA together with omega-conotoxin GVIA (300 nM) pro duced no greater inhibition of the fEPSP than that observed with omega -conotoxin GVIA alone, suggesting that the omega-agatoxin-IVA-sensitiv e and omega-conotoxin MVIIC-sensitive component presents a pharmacolog y similar to the reported Q-type Ca2+ channel. The inhibition produced by omega-conotoxin GVIA and omega-conotoxin MVIIC showed no recovery with prolonged washing (1-2 h) whereas that produced by omega-agatoxin -IVA was slowly reversible. The observation that omega-agatoxin-IVA, w hich does not effect N-type Ca2+ channels (Mintz et al. (1992a) Neuron 9, 85), is capable of completely suppressing the fEPSP suggests that, whilst N-type Ca2+ channels may contribute to normal synaptic transmi ssion at Schaffer collateral-CAl synapses, they are not capable of sup porting transmission when Q-type channels are blocked.