CORRELATION BETWEEN KINETIC AND THERMODYNAMIC COMPLEX-FORMATION CONSTANTS FOR THE INTERACTION OF BIS(AMINE)PALLADIUM(II) WITH INOSINE, INOSINE 5'-MONOPHOSPHATE AND GUANOSINE 5'-MONOPHOSPHATE

The complex-formation equilibria of [Pd(amine)Cl-2] (amine = N,N,N',N' -tetramethyl- or N,N,N',N'-tetraethyl-ethane-1,2-diamine) with inosine , inosine 5'-monophosphate, guanosine and guanosine 5'-monophosphate w ere investigated at different temperatures using a potentiometric tech nique. The stepwise formation constants of the complexes formed in sol ution were calculated using the non-linear least-squares program MINIQ UAD 75. The mode of binding of the DNA unit to the palladium(II) compl exes is discussed. Comparison of the potentiometric results with corre sponding data obtained from kinetic measurements indicates that atom N -7 constitutes the binding site in acidic media and N-1 in basic media . The concentration distribution of the various complex species was ev aluated as a function of pH.