ISOLATION AND CHARACTERIZATION OF A HEMIN-BINDING CELL-ENVELOPE PROTEIN FROM PORPHYROMONAS-GINGIVALIS

Authors
KIM SJ CHU L HOLT SC
Citation
Sj. Kim et al., ISOLATION AND CHARACTERIZATION OF A HEMIN-BINDING CELL-ENVELOPE PROTEIN FROM PORPHYROMONAS-GINGIVALIS, Microbial pathogenesis, 21(1), 1996, pp. 65-70
Citations number
20
Categorie Soggetti
Immunology,Microbiology
Journal title
Microbial pathogenesis → ACNP
ISSN journal
08824010
Volume
21
Issue
1
Year of publication
1996
Pages
65 - 70
Database
ISI
SICI code
0882-4010(1996)21:1<65:IACOAH>2.0.ZU;2-E
Abstract
A 30 kDa (heated 24 kDa) hemin-binding protein whose expression is bot h hemin and iron regulated was identified and purified in Porphyromona s gingivalis 381. A strong hemin-binding function was found by LDS-PAG E and TMBZ staining when cells were grown under hemin (iron)-limited c onditions. N-terminal amino acid sequence analysis of CNBr-digested 24 kDa hemin binding protein revealed that this protein belongs to a new , so far undescribed hemin-binding class of proteins. (C) 1996 Academi c Press Limited