ESTABLISHMENT AND CHARACTERIZATION OF 3 NEW CELL-LINES DERIVED FROM THE ASCITES OF HUMAN OVARIAN CARCINOMAS

Three human cancer cell lines (OC 314, OC 315, and OC 316) were newly established in permanent culture from the ascites of patients with ser ous adenocarcinoma of the ovary. OC 314 was derived from an untreated tumor presenting with ascites at diagnosis; OC 315 was isolated from a neoplasm progressing after cisplatin-containing regimen; and OC 316 w as collected from a patient with pleural metastasis at diagnosis, resi stant to different chemotherapeutic treatments including Taxol. These cell lines were repetitively subcultured once to twice a week through 75-80 passage generations. Tumor cells grew as monolayers and displaye d epithelial-like morphology, consistent with a feature of adenocarcin oma, which was then confirmed by the expressions of cytokeratins and v imentin. The cell Lines proved highly tumorigenic when transplanted in to nude mice, both subcutaneously and intraperitoneally, In addition, the mice inoculated viith subcutaneous OC 316 developed extremely aggr essive tumor, also invading the peritoneum, which correlated with the malignant behavior of the original tumor, Drug sensitivity, evaluated by the MTT Essay, showed that the three cell lines expressed similar s ensitivity to doxorubicin. Responses to cisplatin essentially reported low sensitivity of OC 314 and OC 315 and resistance of OC 316, thus r eflecting the original sensitivity at the clinical level. (C) 1996 Aca demic Press, Inc.