Ja. Chowen et al., SEX STEROID EFFECTS ON THE DEVELOPMENT AND FUNCTIONING OF THE GROWTH-HORMONE AXIS, Cellular and molecular neurobiology, 16(3), 1996, pp. 297-310
1. The secretory pattern of growth hormone (GH) is sexually dimorphic
in the adult rat, However, this difference between the sexes does not
become apparent until after the onset of puberty, suggesting that pube
rtal sex steroids play an important role in the manifestation of this
phenomenon. 2. We have addressed the question as to whether there exis
ts a sexual dimorphism in the hypothalamic neuropeptides that regulate
GH release from the anterior pituitary, i.e., somatostatin (SS) and g
rowth hormone-releasing hormone (GHRH), In addition, we have investiga
ted whether the developmental changes in the GH secretory pattern are
correlated with changes in these neuropeptides. The effect of testoste
rone treatment on SS and GHRH neurons during both the neonatal period
and adulthood have also been studied. 3. We have found that the synthe
tic capacity, as reflected in relative messenger RNA (mRNA) levels, of
both SS and GHRH neurons changes throughout development in both male
and female rats, These mRNA levels are sexually dimorphic at certain t
imes during maturation and can be modulated by changes in testosterone
levels, suggesting that sex steroid modulation of these two neuropept
ide systems could at least partially account for the sexual dimorphism
seen in the adult GH secretory pattern. 4. The neonatal steroid envir
onment has also been suggested to be involved in the generation of the
final adult GH secretory pattern, although the mechanisms underlying
this effect are even less well understood. In support of the hypothesi
s that the neonatal steroid environment plays an important role in org
anizing the GH axis, we have found that the number of GHRH neurons in
the adult brain, as well as their sensitivity to adult steroids, is mo
dulated by neotatal testosterone treatment. The number of SS neurons i
n the periventricular and paraventricular nuclei were not modulated by
neonatal steroids; however, the synthetic capacity of these neurons d
oes appear to be influenced by the neonatal steroid environment. 5. Th
ese studies suggest that both the neonatal and adult sex steroid envir
onments influence the adult GH secretory pattern by modulating GHRH an
d SS neurons.