H. Sugaya et al., MEDIASTINAL LYMPH-NODE CELLS OF ANGIOSTRONGYLUS CANTONENSIS-INFECTED RATS RESPOND TO ANTIGENS AND RELEASE INTERLEUKIN-5 IN-VITRO, International journal for parasitology, 26(5), 1996, pp. 543-551
The kinetics of eosinophil growth and/or survival stimulating factor (
Eo-stimulating factor) production in Angiostrongylus cantonensis-infec
ted rats was assessed by in vitro marrow cultures. When lymphocytes, o
btained from cervical, mediastinal or mesenteric lymph nodes of infect
ed WKAH rats, were cultured with A. cantonensis antigens, Eo-stimulati
ng factor activity was detected in the conditioned media obtained only
from mediastinal lymph node cells at and after 35 days p.i. Eo-stimul
ating factor activity in the conditioned media,vas not detected before
or at 20 days p.i. The kinetics of the factor was, therefore, almost
identical to those of eosinophilia in bone marrow and peripheral blood
. This Eo-stimulating factor activity was inhibited (maximum inhibitio
n = 73%) by anti-mouse IL-5 monoclonal antibody in a dose-dependent fa
shion, indicating that the factor would be, at least in part, identica
l to IL-5. Similar Eo-stimulating factors were also detected in the co
nditioned media which were obtained by stimulating spleen cells from W
istar rats at 35-45 days p.i. with Con A. These data suggest that lymp
hocytes in the mediastinal lymph nodes, local lymph nodes near the adu
lt worm habitat, could play an essential role in induction of eosinoph
ilia in A. cantonensis-infected rats. Copyright (C) 1996 Australian So
ciety for Parasitology. Published by Elsevier Science Ltd.