OPTIMIZED ENZYMATIC-SYNTHESIS OF GERANYL BUTYRATE WITH LIPASE AY FROMCANDIDA-RUGOSA

Response surface methodology (RSM) and five-level, five-variable centr al composite rotatable design (CCRD) were used to evaluate the effects of synthetic variables, such as reaction time (1-9 h), temperature (2 5-65 degrees C), enzyme amount (10-50%), substrate molar ratio of gera niol to tributyrin (1:0.33-1:1), and added water amount (O-20%) on mol ar percent yield of geranyl butyrate, using lipase AY from Candida rug osa. Reaction time and temperature were the most important variables a nd substrate molar ratio had no effect on percent molar conversion. Ba sed on contour plots, optimum conditions were: reaction time 9 h, temp erature 35 degrees C, enzyme amount 50%, substrate molar ratio 1:0.33, and added water 10%. The predicted value was 100% and actual experime ntal value was 96.8% molar conversion. (C) 1996 John Wiley & Sons, Inc .