ISOLATION AND CHARACTERIZATION OF CDNA CLONES CORRESPONDING TO THE GENES EXPRESSED PREFERENTIALLY IN FLORAL ORGANS OF ARABIDOPSIS-THALIANA

Citation
S. Utsugi et al., ISOLATION AND CHARACTERIZATION OF CDNA CLONES CORRESPONDING TO THE GENES EXPRESSED PREFERENTIALLY IN FLORAL ORGANS OF ARABIDOPSIS-THALIANA, Plant molecular biology, 32(4), 1996, pp. 759-765
Citations number
32
Categorie Soggetti
Plant Sciences",Biology
Journal title
ISSN journal
01674412
Volume
32
Issue
4
Year of publication
1996
Pages
759 - 765
Database
ISI
SICI code
0167-4412(1996)32:4<759:IACOCC>2.0.ZU;2-T
Abstract
Seventeen cDNA clones of genes corresponding to mRNAs expressed prefer entially in floral organs of Arabidopsis thaliana were obtained by dif ferential screening of a flower bud cDNA library, and classified into five groups (1A, 17A, 1B, 4B and 5B) by cross-hybridization and restri ction analysis. Sequence analysis revealed that the 1A-1 and 17A-1 clo nes encode vegetative storage proteins (VSPs). The VSP mRNAs were dete cted in a small amount in leaves and increased to a limited level by w ounding. Both 1B-1 and 5B-1 clones were homologous to transmembrane pr otein cDNAs. The protein encoded by 4B-1 clone contained a proline-ric h region, but no homologous proteins were found in databases.