ELECTROTRANSFORMATION OF XANTHOBACTER-AUTOTROPHICUS GJ10 AND OTHER XANTHOBACTER STRAINS

This paper describes the development of an electroporation procedure f or Xanthobacter autotrophicus GJ10. Electroporation has not previously been described for this species. The procedure was optimized with res pect to field strength, pulse time and DNA and cell concentration. Hig h transformation efficiencies of up to 2X10(6) cells transformed per m u g DNA were found. The conditions optimal for the X. autotrophicus GJ 10 were used to electrotransform 9 other Xanthobacter strains 4 of whi ch were transformed with high efficiencies. The electrotransformation procedure described here can be of great help for the genetic availabi lity of Xanthobacter species.