ENHANCEMENT OF THERMOSTABILITY OF LIPASE BY THE SOL-GEL ENTRAPMENT INTO METHYL-SUBSTITUTED ORGANIC SILICATES FORMED ON DIATOMACEOUS-EARTH

Authors
KAWAKAMI K
Citation
K. Kawakami, ENHANCEMENT OF THERMOSTABILITY OF LIPASE BY THE SOL-GEL ENTRAPMENT INTO METHYL-SUBSTITUTED ORGANIC SILICATES FORMED ON DIATOMACEOUS-EARTH, Biotechnology techniques, 10(7), 1996, pp. 491-494
Citations number
7
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
10
Issue
7
Year of publication
1996
Pages
491 - 494
Database
ISI
SICI code
0951-208X(1996)10:7<491:EOTOLB>2.0.ZU;2-#
Abstract
Candida cylindracea lipase was entrapped in methyl-substituted organic silicates formed on kieselguhr such as Celite 545 and Hyflo Super-Gel . Various preparation conditions were optimized to obtain a more therm ostable immobilized lipase. The hybrid gel-entrapped lipase derived fr om an equimolar mixture of trimethylmethoxysilane and tetramethoxysila ne, retained its full activity for ester synthesis up to the temperatu res of 65 degrees C.