P. Ethuin et J. Deconinck, SCALING-UP FOR MASS CULTIVATION OF THE TEMPERATURE-SENSITIVE MUTANT TETRAHYMENA-THERMOPHILA SJ180, Biotechnology techniques, 10(7), 1996, pp. 507-512
Tetrahymena thermophila SJ180 growth was investigated in five complex
media,cultivated in static flasks or in fermenter. In flask, the best
maximum populations were obtained in the yeast extract and glucose med
ium. In fermentor, the best results were observed with the yeast extra
ct and skimmed milk medium Protease secretion was also studied.