A QUICK METHOD FOR PURIFYING BILE SALT-ACTIVATED LIPASES

Authors
MOORE ANJ VAKOS H NEVILLE TAM TONGE P ARYA P BURTON GW
Citation
Anj. Moore et al., A QUICK METHOD FOR PURIFYING BILE SALT-ACTIVATED LIPASES, Biotechnology techniques, 10(7), 1996, pp. 523-528
Citations number
22
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
10
Issue
7
Year of publication
1996
Pages
523 - 528
Database
ISI
SICI code
0951-208X(1996)10:7<523:AQMFPB>2.0.ZU;2-8
Abstract
A simple, one step chromatography procedure of general utility for pur ifying bile salt-activated lipases (BAL; cholesterol esterases) using a cholate affinity column is described. A key feature of the method is the use of a taurocholate concentration gradient in the eluent. The m ethod has been used successfully to purify bovine and porcine BAL from commercial crude sources and BAL from human milk whey.