Th. Tsai et al., DETERMINATION OF WOGONIN IN RAT PLASMA BY LIQUID-CHROMATOGRAPHY AND ITS PHARMACOKINETIC APPLICATION, Planta medica, 62(3), 1996, pp. 263-266
A high-performance liquid chromatographic method has been developed fo
r the determination of wogonin in the rat plasma. After addition of an
internal standard (clomipramine), plasma was deproteinized by acetoni
trile and centrifuged for sample clean-up. The supernatant was separat
ed by a reversed-phase column chromatography, identified by a photodio
de-array detector, and quantified by ultraviolet detection at a wavele
ngth of 275 nm. Acetonitrile-water-diethylamine (50:50:0.1, v/v/v, pH
3.0 adjusted by orthophosphoric acid) was used as the mobile phase. Th
e method was applied to the pharmacokinetic study of wogonin in rats a
fter a dose of 5 mg/kg by intravenous administration. A biphasic pheno
menon with a rapid distribution followed by a slower elimination phase
was observed from the plasma concentration time profile.