ANALYSIS OF SEVERAL IRIDOID AND INDOLE PRECURSORS OF TERPENOID INDOLEALKALOIDS WITH A SINGLE HPLC RUN

An isocratic HPLC system is described which allows the separation of t he iridoid and indole precursors of terpenoid indole alkaloids, which are present in a single crude extract. The system consists of a column of LiChrospher 60 RP select 6 5 mu m, 250 x 4mm (Merck) with an eluen t of 1 % formic acid-acetonitrile-trichloroacetic acid (100:10:0.25, v :v:w) at a flow of 1.2 ml/min. In the suspension cultures of Catharant hus roseus secologanin and tryptophan were detected. In the cultures o f Tabernaemontana divaricata loganin, tryptophan, and tryptamine accum ulated.