From Italian plants of Withania somnifera six withanolides were isolat
ed, whose structures allowed us to assign the Italian race of W. somni
fera to the Israel chemotype III. In vitro cultures of Italian W. somn
ifera under different conditions were obtained, as well as infection b
y Agrobacterium rhizogenes. HPLC analysis of in vitro derived tissues
showed low contents of withanolides.