CHARACTERISTICS OF PHOSPHOLIPASE-D IN REVERSE MICELLES OF TRITON X-100 AND PHOSPHATIDYLCHOLINE IN DIETHYL-ETHER

Authors
SUBRAMANI S DITTRICH N HIRCHE F ULBRICHHOFMANN R
Citation
S. Subramani et al., CHARACTERISTICS OF PHOSPHOLIPASE-D IN REVERSE MICELLES OF TRITON X-100 AND PHOSPHATIDYLCHOLINE IN DIETHYL-ETHER, Biotechnology letters, 18(7), 1996, pp. 815-820
Citations number
14
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
18
Issue
7
Year of publication
1996
Pages
815 - 820
Database
ISI
SICI code
0141-5492(1996)18:7<815:COPIRM>2.0.ZU;2-W
Abstract
Phospholipase D, from cabbage, is active in reverse micelles formed fr om its substrate phosphatidylcholine and Triton X-100 in diethyl ether . The activity is optimum at w(0)=12.5. The increase of the molar rati o of Triton X-100/substrate from 1:4 to 2:1 results in an activity dec rease by 25%. At 136 mM Triton X-100 the K-M value in reverse micelles is 136 mM, whereas it is 0.40 mM in the aqueous system containing SDS .