A. Amicucci et al., IDENTIFICATION OF ECTOMYCORRHIZAE FROM TUBER SPECIES BY RFLP ANALYSISOF THE ITS REGION, Biotechnology letters, 18(7), 1996, pp. 821-826
Polymorphisms of a ribosomal DNA I region (ITS) have been analysed usi
ng a specific pair of primers, in order Co type fruitbodies and ectomy
corrhizae of different truffle species. The identification of ectomyco
rrhizae was obtained by digestion of the PCR products using restrictio
n enzymes. The results show that the strategy used is both suitable an
d sensitive to characterize the symbiotic fungi from few mycorrhized r
oot tips.