PRODUCTION OF 1,3-PROPANEDIOL BY CLOSTRIDIUM-BUTYRICUM DSM-5431 AND PRODUCT TOLERANT MUTANTS IN FEDBATCH CULTURE - FEEDING STRATEGY FOR GLYCEROL AND AMMONIUM

A fedbatch strategy was developed coupling the feeding of the two inhi bitory substrates glycerol and ammonium to alkali consumption. A conti nuous, automated substrate addition was achieved responding directly t o the needs of the culture. Thus substrate concentrations were kept on a constant low, but non limiting level. The feeding was applied for t he cultivation of Clostridium butyricum DSM 5431 and mutants with incr eased product tolerance. Compared to fedbatch cultivations with interm ittent feeding cultivation times were considerably shortened.