ALTERATION OF INTRAERYTHROCYTE PROTEOLYTIC DEGRADATION OF HEMOGLOBIN DURING HODGKINS-DISEASE

The erythrocyte lysate samples obtained from 10 healthy donors (aged o f 23 +/- 12 years) and 16 patients with Hodgkin's disease (aged of 39 +/- 25 years) with the following histological types: 12 mixed cellular ity and 4 nodular sclerosis, were studied, Patients with Hodgkin's dis ease (HD) were randomly selected before, during or after the completio n of combined chemotherapy. A comparative analysis of peptide componen ts of erythrocyte lysate samples of HD patients and healthy donors was carried out. The amino acid sequences of 4 peptides, corresponding to fragments 1-33,1-32,1-31 and 1-30 of human hemoglobin (Hb) alpha-chai n were determined. Increase of the content of two fragments correspond ing to 1-31 and 1-32 amino acid residues of alpha-globin were detected for HD patients. The link between the normal process of proteolytic d egradation and those occurring during HD is proposed. The possibility of using the identified alterations recorded during HD diagnosis is di scussed.