Am. Gonchar et Vl. Auslender, IMMOBILIZATION OF BACTERIAL PROTEASES ON WATER-SOLVED POLYMER BY MEANS OF ELECTRON-BEAM, Radiation physics and chemistry, 48(6), 1996, pp. 795-797
Citations number
9
Categorie Soggetti
Nuclear Sciences & Tecnology","Chemistry Physical","Physics, Atomic, Molecular & Chemical
Possibility of electron beam usage for proteases' immobilization on 1,
4-polyalkylene oxide (1,4-PAO) was studied to obtain biologically acti
ve complex for multi-purpose usage. It is shown that immobilization of
Bacillus Subtilis protease takes place due to free-radical linking of
enzyme and carrier with formation of mycellium-like structures. Immob
ilization improves heat resistance of enzyme up to 60 degrees C withou
t substrate and up to 80 degrees C in presence of substrate, widens ra
nge of pH activity in comparison with non-immobilized forms. Immobiliz
ed proteases do not contain peroxides or long-live radicals. Our resul
ts permitted to create technologies for production of medical and vete
rinary preparations, active components for wool washing agents and lea
ther fabrication technology. Copyright (C) 1996 Elsevier Science Ltd