Gc. Gussenhoven et al., LEPTO DIPSTICK, A DIPSTICK ASSAY FOR DETECTION OF LEPTOSPIRA-SPECIFICIMMUNOGLOBULIN-M ANTIBODIES IN HUMAN SERA, Journal of clinical microbiology, 35(1), 1997, pp. 92-97
We studied a dipstick assay for the detection of Leptospira-specific i
mmunoglobulin M (IgM) antibodies in human serum samples. A high degree
of concordance was observed between the results of the dipstick assay
and an IgM enzyme-linked immunosorbent assay (ELISA), Application of
the dipstick assay for the detection of acute leptospirosis enabled th
e accurate identification, early in the disease, of a high proportion
of the cases of leptospirosis. Analysis of a second serum sample is re
commended, in order to determine seroconversion or increased staining
intensity. All serum samples from the patients who were confirmed to b
e positive for leptospirosis by either a positive microscopic agglutin
ation test or a positive culture but were found to be negative by the
dipstick assay were also judged to be negative by the IgM ELISA or rev
ealed borderline titers by the IgM ELISA. Some cross-reactivity was ob
served for sera from patients with diseases other than leptospirosis,
and this should be taken into account in the interpretation of test re
sults. The dipstick assay is easy to perform, can be performed quickly
, and requires no electricity or special equipment, and the assay comp
onents, a dipstick and a staining reagent, can be stored for a prolong
ed period without a loss of reactivity, even at elevated temperatures.