2ND TRANSPLANTATION WITH CD34(-MARROW CELLS SELECTED FROM A 2-LOCI HLA-MISMATCHED SIBLING FOR A PATIENT WITH CHRONIC MYELOID-LEUKEMIA() BONE)

A 43-year-old man with chronic myeloid leukaemia underwent a second tr ansplant with CD34(+) bone marrow cells selected from his two-loci HLA -mismatched sibling after rejection of the first graft from an HLA-mat ched unrelated donor. By immunomagnetic positive selection, CD34(+) ma rrow cells at 0 . 95 x 10(6)/kg with 97% purity and CD3(+) T lymphocyt es at 1 . 3 x 10(4)/kg were collected and transplanted. Engraftment wa s confirmed to be of CD34(+) cell-donor origin. The patient developed only grade I acute graft-versus-host disease (GVHD) and no chronic GVH D to date. These observations suggest that allogeneic CD34(+) bone mar row cells are capable of reconstituting haemopoiesis and that CD34(+) selection could be applicable to T-cell depletion.