The chromosomes of Bufo crucifer, B. ictericus, and B. pamacnemis were
studied by conventional staining as well as with C banding and NOR te
chniques. These species have a diploid number of 2n = 22 and identical
karyotypes, composed of metacentric and submetacentric chromosomes. T
he C banding patterns and NOR data indicate that these species of Bufo
are not differentiated by the distribution and amount of constitutive
heterochromatin or the position of the nucleolar organizer regions.