Five AI-bulls (5.5-6 years of age) fitted with permanent vena cava or
aorta abdominalis canulae were subjected to an one week lasting food r
eduction used as a metabolic stress test. Frequent bleedings were perf
ormed every 30 minutes for 10 hour periods from 5 days prior to until
35 days after start of the challenge. Reproductive and metabolic hormo
nes (LH, testosterone, growth hormone, insulin and insulin like growth
factor-I) were determined by radio-immunoassays.