Ab. Harrison et Wb. Betts, ASSAY OF BACTERIAL AND FUNGAL ACTIVITY IN DIESEL CONTAMINATED SOIL USING A C-14 GLUCOSE-UTILIZATION METHOD, Letters in applied microbiology, 23(1), 1996, pp. 43-46
Changes in the relative metabolism of soil bacteria and fungi followin
g contamination with diesel were assessed using a modified substrate-i
nduced respiration (SIR) method including selective antibiotic inhibit
ion. (CO2)-C-14 release from radiolabelled glucose was used as an indi
cation of population activity. In a Sandy Gley Soil with no history of
contamination, the population activity shifted from 38 +/- 4% (bacter
ial) : 62 +/- 4% (fungal) to 73 +/- 4% (bacterial) : 27 +/- 4% (fungal
) after treatment with diesel.