Ra. Bicknell et Nk. Borst, ISOLATION OF REDUCED GENOTYPES OF HIERACIUM-PILOSELLA USING ANTHER CULTURE, Plant cell, tissue and organ culture, 45(1), 1996, pp. 37-41
Plants of reduced ploidy were derived from anther tissue of a pentaplo
id, apomictic biotype of Hieracium pilosella (mouse-ear hawkweed). Cal
lusing was observed from both the somatic and generative tissue of the
anther. Capitulum diameter was used as a correlative character to det
ermine the best stage for harvesting the tissue. The highest degree of
callusing coincided with the early uninucleate stage of microsporogen
esis. Regenerants included tetraploids, triploids and two classes of a
neuploids. Segregation for apomixis was observed among the tetraploid
regenerants, an indication of the dominant inheritance of apomixis in
this species.