PURIFICATION AND CHARACTERIZATION OF CATIONIC TRYPSIN FROM THE PANCREAS OF THE ARABIAN CAMEL (CAMELUS-DROMEDARIUS)

Cationic trypsin was purified from a crude extract of camel pancreas i n a 90% yield by a two step procedure consisting of anion-exchange and affinity chromatographies. The purified enzyme was homogeneous on nat ive and SDS gel electrophoresis. Its molecular mass was estimated to b e 22 kDa. Camel cationic trypsin was seen to be similar to its bovine counterpart in substrate specificity, susceptibility to inhibition, pH optimum and catalytic stability. Copyright (C) 1996 Elsevier Science Inc.