J. Bruch et al., MAPPING OF TYPE-I LOCI FROM HUMAN-CHROMOSOME-7 REVEALS SEGMENTS OF CONSERVED SYNTENY ON PIG CHROMOSOME-3, CHROMOSOME-9, AND CHROMOSOME-18, Cytogenetics and cell genetics, 73(3), 1996, pp. 164-167
We have mapped in the pig (Sus scrofa) the genes for zona pellucida gl
ycoprotein 3 (ZP3), erythropoietin (EPO), and malate dehydrogenase 2 (
MDH2) by somatic cell hybrid analysis in the pig genome. Previously, t
he gene for the T-cell receptor beta cluster (TCRB) was assigned to SS
C 18 and that. for interleukin 6 (IL6) to SSC 9. However, statistical
analysis of mapping data for ZP3, EPO, and MDH2 did not discriminate b
etween SSC 9 and SSC 3. Porcine-specific PCR primers for ZP3 and IL6 w
ere used to isolate probes from porcine YAC libraries. These two genes
were mapped by FISH to porcine chromosome regions 3pter-->p15 (ZP3) a
nd 9q14-->q15 (IL6). Comparative mapping considerations permit the ten
tative assignment of porcine EPO and MDH2 to 3pter-->p15.