MICROCHIP ENCODED COMBINATORIAL LIBRARIES - GENERATION OF A SPATIALLYENCODED LIBRARY FROM A POOL SYNTHESIS

An encoding method which provides ready access to the structure of ind ividual compounds in a combinatorial library of small organic molecule s has been developed. Glass-encased microchips each of which contain a unique binary encoded ID which can be scanned and recorded using radi ofrequency (RF) were added to individual tea bags containing polystyre ne polymer functionalized with a Wang linker. The tea bags were subjec ted to a three-step synthesis. Ar each stage of the synthesis, the mic rochips were RF-scanned and the unique LD's were recorded. After the s ynthesis was complete, each tea bag was introduced to individual wells of a microtiter plate and the products were deblocked from the polyme r. The histogram of the LD for each well was then used to assign the s tructure of every product in the Library. A library of 64 compounds wa s thus synthesized using a pooled compound strategy, affording a posit ionally encoded discrete library.