COLLAGEN TYPE-I AND TYPE-III LOCALIZATION BY IN-SITU HYBRIDIZATION AND IMMUNOHISTOCHEMISTRY IN THE PARTIALLY OBSTRUCTED YOUNG-RABBIT BLADDER

Purpose: The objective of the study was to investigate the alteration of collagen expression patterns at the transcription and translation l evels during partial outlet obstruction and to compare changes in coll agen types I and III gene expression,and protein deposition during ear ly and subacute phases of obstruction. Materials and Methods: Followin g 1, 2, 7, 14 and 21 days; of partial bladder obstruction in 2-week-ol d rabbits bladder tissues were evaluated for collagen-types I and III messenger ribonucleic acid localization by in situ hybridization and f or protein localization by immunohistochemical study. Results: There i s up regulation of collagen types I and III gene expression after obst ruction. Up regulation of collagen gene expression immediately after o bstruction within the lamina propria and the message moves into the mu scular layer as obstruction progresses. Protein deposition for both co llagen types is analogous to that of gene expression. Conclusions: Up regulation of collagen types I and III gene expression, and increased protein deposition after partial obstruction demonstrate that the alte ration in collagen synthesis is at least partially transcription regul ated. This alteration-tarts from superficial layers of the bladder wal l and becomes deeper as the pathological process progresses.