MOLECULAR CHARACTERIZATION OF LACTOBACILLUS-CASEI STRAINS

Authors
FERRERO M CESENA C MORELLI L SCOLARI G VESCOVO M
Citation
M. Ferrero et al., MOLECULAR CHARACTERIZATION OF LACTOBACILLUS-CASEI STRAINS, FEMS microbiology letters, 140(2-3), 1996, pp. 215-219
Citations number
11
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
140
Issue
2-3
Year of publication
1996
Pages
215 - 219
Database
ISI
SICI code
0378-1097(1996)140:2-3<215:MCOLS>2.0.ZU;2-#
Abstract
Three different molecular techniques were used to characterize bacteri al strains isolated from dairy products and phenotypically classified as Lactobacillus casei. Some strains were taxonomically identified as Lb. casei and Lb. paracasei using 23S rRNA-targeted probes specific fo r Lb. casei and Lb. paracasei. Chromosomal DNA was analyzed by pulsed- field gel electrophoresis. Lb. paracasei strains had a variable number of SfiI fragments harboring rRNA genes, while Lb. casei strains showe d more homogeneous patterns. The chromosome size of Lb. paracasei ATCC 334 was evaluated to be 2.17 Mb.