N. Takamatsumatsushita et al., IMMUNOGENICITY OF ACTINOBACILLUS-ACTINOMYCETEMCOMITANS SEROTYPE B-SPECIFIC POLYSACCHARIDE-PROTEIN CONJUGATE, Oral microbiology and immunology, 11(4), 1996, pp. 220-225
Citations number
38
Categorie Soggetti
Immunology,Microbiology,"Dentistry,Oral Surgery & Medicine
To enhance the immunogenicity of capsular-like serotype b-specific pol
ysaccharide antigen (SPA) of Actinobacillus actinomycetemcomitans, the
purified antigen was coupled with bovine serum albumin via an adipic
acid hydrazide functional group. The conjugate (SPA-bovine serum album
in) or the native SPA was administered subcutaneously or intranasally
to BALB/c mice. Neither subcutaneous immunization with SPA emulsified
in Freund adjuvant nor intranasal immunization with SPA and cholera to
xin B subunit elicited any antibody responses to the polysaccharide an
tigen. High serum immunoglobulin M (IgM) and IgG responses to SPA were
induced by subcutaneous immunization with SPA-bovine serum albumin in
Freund adjuvant. Serum and salivary IgA responses to SPA in addition
to IgM and IgG responses were induced by intranasal immunization with
the conjugate and cholera toxin B subunit. To investigate the function
al activity of SPA-specific antibodies, the opsonic activity of sera f
rom BALB/c mice immunized with the conjugate was assessed by chemilumi
nescence assay using human polymorphonuclear leukocytes. Murine antise
ra efficiently opsonized A. actinomycetemcomitans serotype b in the as
say, suggesting that antibodies to SPA of the organism might serve as
a protective role.