UNSTABLE SYSTEM SN-49 IN DROSOPHILA-MELAN OGASTER - ANALYSIS BY BLOT-HYBRIDIZATION AND POLYMERASE CHAIN-REACTION

A set of 40 strains of alleles of different phenotype and mutation fre quency, derived from unstable system sn(49) of Drosophila melanogaster , was obtained. A mutation in the singed gene or the formation of a vi sible de novo mutation in the studied X chromosome served as the crite rion for strain isolation. Total DNA was isolated from the obtained st rains and used for blot-hybridization and polymerase chain reaction (P CR). Rearrangements were found within the first intron of the singed g ene. Zn the first intron of an allelic derivative of this unstable sys tem, an inserted sequence of at least 4 kb in length was found. Using PCR primers from this intron, fragment length polymorphism in differen t derivatives was revealed.