A. Higuchi et al., REJECTION OF SINGLE-STRANDED AND DOUBLE-STRANDED DNA BY POROUS HOLLOW-FIBER MEMBRANES, Journal of membrane science, 116(2), 1996, pp. 191-197
The permeation of DNA through regenerated cellulose hollow fibers that
have a mean pore diameter of 15 nm was investigated in the absence of
protein at a transmembrane pressure of 200 mmHg at 25 degrees C. The
membranes can reject both double stranded DNA and single stranded DNA
to some extent when the DNA concentration of the feed solution is 10-1
00 ppm. It was found that single stranded DNA can permeate through the
membranes preferentially over double stranded DNA. This is explained
by the higher flexibility of the single stranded DNA over the double s
tranded DNA and is not mainly due to a different distribution of molec
ular weights between the single stranded DNA and the double stranded D
NA. The circular dichroism spectra of feed and permeate solutions sugg
est that the DNA was not denatured by permeation through the membranes
.