ANTAGONISTIC EFFECT OF AMPHOTERICIN-B ON CARBOPLATIN ANTITUMOR-ACTIVITY IN HUMAN OSTEOSARCOMA XENOGRAFTS

Amphotericin a (AmB), a polyene antifungal antibiotic, has been shown to potentiate the cytotoxic effect of different chemotherapeutic drugs in vivo and in vitro. The purpose of this study was to determine whet her AmB could enhance the carboplatin antitumor activity in a human os teosarcoma xenograft model, Nude mice, bearing s.c. transplanted osteo sarcoma xenografts, received i.p. an injection of AmB (5 mg/kg) 6 h pr ior to carboplatin (20 mg/kg) or each of the drugs separately, The eff ect of treatment was assessed by analyzing tumor growth delay and T/C ratio, Carboplatin clearly reduced tumor growth when administered alon e, However, an unexpected interaction was seen where AmB significantly decreased the antitumor effect of carboplatin, The present results co ntradict some earlier in vitro studies and indicate the complexity of this interaction in vivo, Hence, it seems that interactive phenomena i n one experimental model, and especially with regard to AmB, cannot be universally applied to all experimental situations.