Af. Goguel et al., MAPPING OF 22 YACS ON HUMAN-CHROMOSOMES BY FISH USING YEAST DNA ALU-PCR PRODUCTS FOR COMPETITION, Annales de genetique, 39(2), 1996, pp. 64-68
Chromosomal assignment and analysis of chimerism of 22 YACs was perfor
med by FISH, Probes were obtained by PCR amplification of the human YA
C inserts with Alu primers, Maximum amplification of various inter-Alu
elements was obtained when the primer annealing temperature was below
the optimal temperature needed for high specificity, In these conditi
ons, yeast DNA contributed to the amplification of various Alu-PCR pro
ducts and, since strong competition was required for the suppression o
f all Alu sequences, yeast Alu-PCR products fulfilled this purpose eff
iciently.