MAPPING OF 22 YACS ON HUMAN-CHROMOSOMES BY FISH USING YEAST DNA ALU-PCR PRODUCTS FOR COMPETITION

Citation
Af. Goguel et al., MAPPING OF 22 YACS ON HUMAN-CHROMOSOMES BY FISH USING YEAST DNA ALU-PCR PRODUCTS FOR COMPETITION, Annales de genetique, 39(2), 1996, pp. 64-68
Citations number
19
Categorie Soggetti
Genetics & Heredity
Journal title
ISSN journal
00033995
Volume
39
Issue
2
Year of publication
1996
Pages
64 - 68
Database
ISI
SICI code
0003-3995(1996)39:2<64:MO2YOH>2.0.ZU;2-0
Abstract
Chromosomal assignment and analysis of chimerism of 22 YACs was perfor med by FISH, Probes were obtained by PCR amplification of the human YA C inserts with Alu primers, Maximum amplification of various inter-Alu elements was obtained when the primer annealing temperature was below the optimal temperature needed for high specificity, In these conditi ons, yeast DNA contributed to the amplification of various Alu-PCR pro ducts and, since strong competition was required for the suppression o f all Alu sequences, yeast Alu-PCR products fulfilled this purpose eff iciently.