REGULATION OF ION CHANNELS IN SMOOTH MUSCLES BY CALCIUM

Intracellular Ca2+ concentration ([Ca2+](i)) plays a central role in r egulating tone and contractility of smooth muscle cells. In contrast t o the ''classic'' model of electromechanical coupling where membrane p otential determines [Ca2+](i), it is now well established that [Ca2+]( i) in turn may also affect membrane potential by modulating open proba bilities of ion channels. Activation by [Ca2+](i) of large-conductance K+ channels, Cl- channels, and nonselective cation channels has been described, as well as block of delayed rectifier K+ channels by [Ca2+] (i) and [Ca2+](i)-induced inactivation of Ca2+ channels. Therefore, a network consisting of positive- and negative-feedback loops regulates [Ca2+](i) as well as membrane potential. In this context, we review th e properties of Ca2+-dependent ion channels and their functional role in vascular and visceral smooth muscles. Any alteration of the ''Ca2sensitivity'' of ion channels is expected to have a profound effect on the reciprocal relationship between membrane potential and [Ca2+](i). Already several molecular factors determining Ca2+ sensitivity of Ca2 +-activated K+ channels have been identified. We provide a working def inition for Ca2+ sensitivity.