FUNCTIONAL NMDA RECEPTORS ARE TRANSIENTLY ACTIVE AND SUPPORT THE SURVIVAL OF PURKINJE-CELLS IN CULTURE

Conflicting evidence exists concerning the activity of NMDA receptors (NMDARs) in cerebellar Purkinje cells and their possible functions. To investigate the activity of NMDARs, we used whole-cell recording on i mmunocytochemically identified Purkinje cells in primary culture. In a ddition, we used mice with a disrupted NMDAR1 gene that lack functiona l NMDARs (NR1-/-) to assess the physiological role of NMDARs. In cultu res from normal mice, NMDA-mediated currents were detected in all iden tified Purkinje cells at 4 d in vitro (div). After 14 d, however, NMDA responses were reduced in amplitude, whereas the responses to kainate and glutamate increased steadily in amplitude. In addition, the NMDA- induced current displayed a pronounced desensitization at these later stages; peak current declined to zero during steady application of NMD A. At 7 div, the number of surviving Purkinje cells was less in cultur es treated with NMDA antagonists, and their survival was dose-dependen t. Purkinje cell survival was correspondingly poorer in cultures from the NR1-/- mice than in wild-type controls, suggesting that NMDAR acti vity enhances the survival of Purkinje cells in vitro. The addition of moderate doses of NMDA promoted the survival of wild-type Purkinje ce lls in the presence of tetrodotoxin. Feeder layers of cerebellar granu le cells derived from wild-type or NR1-/- mice promoted survival of Pu rkinje cells to a similar degree, suggesting that the NMDAR in Purkinj e cells, but not in other cells, is directly involved in Purkinje cell viability. The results demonstrate that NMDARs transiently produce me mbrane current in Purkinje cells and may serve as one of the epigeneti c factors that support the survival of Purkinje cells in vitro.