Fa. Anania et al., ACTIVATION BY ACETALDEHYDE OF THE PROMOTER OF THE MOUSE ALPHA(2)(I) COLLAGEN GENE WHEN TRANSFECTED INTO RAT ACTIVATED STELLATE CELLS, Archives of biochemistry and biophysics, 331(2), 1996, pp. 187-193
The effect of acetaldehyde in activating the mouse alpha(2)(I) collage
n promoter in transiently transfected rat activated stellate cells and
the possible mediating effect of transforming growth factor beta(1) (
TGF beta(1)) on type I collagen gene expression were determined. Aceta
ldehyde and TGF beta(1), each had a similar effect in activating the w
ild-type promoter, but failed to activate the promoter with a -352 to
-104 deletion, or the promoter containing a 3-bp substitution between
-305 and -303 in the putative nuclear factor I (NF-I) binding site. Th
e effects of acetaldehyde and TGF beta(1) are therefore mediated by a
similar factor or factors that bind to the NF-I consensus sequence wit
hin the region -352 to -104. Additional factors may also play a role i
n the effects of acetaldehyde and TGF beta(1), which have similar effe
cts on the wild-type promoter, but become additive in activating the p
romoter with a more distal deletion containing a cis-repressor element
. Pretreatment of activated stellate cells with antibodies to TGF beta
(1) suppressed the effect of acetaldehyde in increasing the alpha(1)(I
) collagen message, indicating that TGF beta(1) mediates the effect of
the acetaldehyde-induced increase in the expression of the alpha(1)(I
) collagen gene which also contains NF-I binding sites. (C) 1996 Acade
mic Press, Inc.