The present investigation was conducted to examine the effects of para
thyroid hormone (PTH) and parathyroid hormone related peptide (PTHrP)
on renal calbindin-D28k in rats. Four soups of studies were performed:
(1) parathyroidectomy (PTX) or a sham operation followed by infusion
of 1,25-dihydroxyvitamin D (1,25[OH]D-2) or vehicle; (2) infusions of
PTH(1-34), PTH(1-84), 1,25(OH),D, or vehicle; (3) infusion of PTHrP(1-
34), PTHrP(1-86), PTH(1-34), or vehicle; and (4) injections of calcium
or vehicle. PTX reduced renal calbindin-D28k levels even when plasma
concentrations of 1,25(OH)(2)D were kept constant by infusion of 1,25(
OH)(2)D. Infusions of PTH(1-34), PTH(1-84), and 1,25(OH)(2)D all incre
ased renal calbindin-D28k and plasma calcium, whereas PTHrP(1-34) and
PTHrP(1-86) increased renal calbindin-D28k before an increase of plasm
a calcium took place. Hypercalcemia induced by the injection of calciu
m did not affect the levels of renal calbindin-D28k The present data s
uggest that PTH and PTHrP exert a direct effect on renal calbindin-D28
k, which is not mediated by changes of 1,25(OH)(2)D or calcium.