CLONING AND SEQUENCING OF CDNAS ENCODING THE HUMAN SPERM PROTEIN, SP17

Authors
LEA IA RICHARDSON RT WIDGREN EE ORAND MG
Citation
Ia. Lea et al., CLONING AND SEQUENCING OF CDNAS ENCODING THE HUMAN SPERM PROTEIN, SP17, Biochimica et biophysica acta, N. Gene structure and expression, 1307(3), 1996, pp. 263-266
Citations number
4
Categorie Soggetti
Biology,Biophysics,"Biothechnology & Applied Migrobiology
Journal title
Biochimica et biophysica acta, N. Gene structure and expression → ACNP
ISSN journal
01674781
Volume
1307
Issue
3
Year of publication
1996
Pages
263 - 266
Database
ISI
SICI code
0167-4781(1996)1307:3<263:CASOCE>2.0.ZU;2-Y
Abstract
In the present study we have cloned and sequenced two testis-specific cDNAs (1.3 kb and 1.6 kb) encoding a human sperm protein, designated H Sp17. Each cDNA gave rise to identical protein sequences and differed only in the 5' untranslated region. The predicted amino-acid sequence revealed a protein of 17.5 kDa which exhibited a high degree of homolo gy with both rabbit and mouse Sp17. Analysis of native and recombinant Sp17 by SDS-PAGE has shown the apparent molecular weight of the prote in to be 24.5 kDa.