CHARACTERIZATION OF A NEW PEPTIDE FROM TITYUS-SERRULATUS SCORPION-VENOM WHICH IS A LIGAND OF THE APAMIN-BINDING SITE

Authors
LEGROS C OUGHUIDENI R DARBON H ROCHAT H BOUGIS PE MARTINEAUCLAIRE MF
Citation
C. Legros et al., CHARACTERIZATION OF A NEW PEPTIDE FROM TITYUS-SERRULATUS SCORPION-VENOM WHICH IS A LIGAND OF THE APAMIN-BINDING SITE, FEBS letters, 390(1), 1996, pp. 81-84
Citations number
23
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
390
Issue
1
Year of publication
1996
Pages
81 - 84
Database
ISI
SICI code
0014-5793(1996)390:1<81:COANPF>2.0.ZU;2-W
Abstract
A new ligand (Ts k) of the apamin binding site on rat brain synaptosom es (K-0.5 = 300 PM) was purified and characterized from the venom of T ityus serulatus. It is a polypeptide toxin of 35 amino acid residues, with three disulfide bridges, Its cDNA was amplified from a venom glan d cDNA library and the nucleotide sequence determined, A model of Ts I c was constructed by amino acid replacement using charybdotoxin struct ure as determined by H-1 nuclear magnetic resonance as starting model.