ASSIGNMENT OF MARKERS BY USING POLYMERASE CHAIN-REACTION ON POOLS OF SWINE FLOW-SORTED CHROMOSOMES

Gene chromosomal assignment can be realized not only by somatic hybrid panels but also by spot-blot hybridization or polymerase chain reacti on (PCR) of flow-sorted chromosomes. We propose a swine chromosome ass ignment strategy by PCR amplification on pooled chromosomal DNA, which allows assignment despite possible chromosomal contamination during s orting. Each pool contains three different chromosomes, each chromosom e being present in one or two pools. We present concordant results obt ained for eight markers already mapped to different swine chromosomes and we assign the somatostatin gene to chromosome 13, a new marker in the pig genome.