CHARACTERIZATION AND LINKAGE MAPPING OFTEN SHEEP MICROSATELLITE MARKERS DERIVED FROM A SHEEP X HAMSTER-CELL HYBRID

Authors
LUMSDEN JM LORD EA MONTGOMERY GW
Citation
Jm. Lumsden et al., CHARACTERIZATION AND LINKAGE MAPPING OFTEN SHEEP MICROSATELLITE MARKERS DERIVED FROM A SHEEP X HAMSTER-CELL HYBRID, Animal genetics, 27(3), 1996, pp. 203-206
Citations number
8
Categorie Soggetti
Genetics & Heredity","Veterinary Sciences
Journal title
Animal genetics → ACNP
ISSN journal
02689146
Volume
27
Issue
3
Year of publication
1996
Pages
203 - 206
Database
ISI
SICI code
0268-9146(1996)27:3<203:CALMOS>2.0.ZU;2-3
Abstract
A cosmid library has been constructed from a sheep x hamster cell hybr id containing sheep chromosome t(1), rob (6;24). Clones containing she ep DNA were identified by hybridizing to a total sheep genomic DNA pro be. Small fragments (<500 bp) containing (AC)(n) microsatellites were subcloned and sequenced. Ten microsatellite markers were characterized and six were mapped back to chromosomes 6 and 24. The remaining micro satellites mapped to chromosome 26, which was shown to be present in a small proportion of cells of the cell line.